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crispr plasmid vector px459 v2 0 espcas9  (Addgene inc)


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    Structured Review

    Addgene inc crispr plasmid vector px459 v2 0 espcas9
    Crispr Plasmid Vector Px459 V2 0 Espcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+crispr+system+plasmid/eSpCas9(1%2E1)+(Plasmid+%2371814)/pm41762386-36-9-14
    Average 96 stars, based on 205 article reviews
    crispr plasmid vector px459 v2 0 espcas9 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Sequencing:

    Article Title: RGS11-CaMKII complex mediated redox control attenuates chemotherapy-induced cardiac fibrosis.
    Article Snippet: Guide RNA (gRNA) targeting human RGS11 gene exon 15 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Article Title: G protein β5-ATM complexes drive acetaminophen-induced hepatotoxicity
    Article Snippet: Guide RNA (gRNA) targeting human GNB5 gene exon2 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods [ ] and confirmed via sequencing. .. Briefly, 10 μg of Px459 plasmid was digested with 5 μl (10 units) of BbsI restriction enzyme (NEB) for 5 h at 37 °C, run on 1% agarose gel and the digested product was then eluted with a gel extraction kit (Qiagen).

    Article Title: RGS11-CaMKII complex mediated redox control attenuates chemotherapy-induced cardiac fibrosis
    Article Snippet: Guide RNA (gRNA) targeting human RGS11 gene exon 15 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Article Title: A RGS7-CaMKII complex drives myocyte-intrinsic and myocyte-extrinsic mechanisms of chemotherapy-induced cardiotoxicity
    Article Snippet: Recipient cells were collected for further processing after 12-16 h. Generation of RGS7 KO AC-16 cells using CRISPR/Cas9 Guide RNA (gRNA) targeting human RGS7 gene exon17 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods as we previously described(2) and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Clone Assay:

    Article Title: RGS11-CaMKII complex mediated redox control attenuates chemotherapy-induced cardiac fibrosis.
    Article Snippet: Guide RNA (gRNA) targeting human RGS11 gene exon 15 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Article Title: G protein β5-ATM complexes drive acetaminophen-induced hepatotoxicity
    Article Snippet: Guide RNA (gRNA) targeting human GNB5 gene exon2 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods [ ] and confirmed via sequencing. .. Briefly, 10 μg of Px459 plasmid was digested with 5 μl (10 units) of BbsI restriction enzyme (NEB) for 5 h at 37 °C, run on 1% agarose gel and the digested product was then eluted with a gel extraction kit (Qiagen).

    Article Title: RGS11-CaMKII complex mediated redox control attenuates chemotherapy-induced cardiac fibrosis
    Article Snippet: Guide RNA (gRNA) targeting human RGS11 gene exon 15 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Article Title: A RGS7-CaMKII complex drives myocyte-intrinsic and myocyte-extrinsic mechanisms of chemotherapy-induced cardiotoxicity
    Article Snippet: Recipient cells were collected for further processing after 12-16 h. Generation of RGS7 KO AC-16 cells using CRISPR/Cas9 Guide RNA (gRNA) targeting human RGS7 gene exon17 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods as we previously described(2) and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    CRISPR:

    Article Title: RGS11-CaMKII complex mediated redox control attenuates chemotherapy-induced cardiac fibrosis.
    Article Snippet: Guide RNA (gRNA) targeting human RGS11 gene exon 15 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Article Title: G protein β5-ATM complexes drive acetaminophen-induced hepatotoxicity
    Article Snippet: Guide RNA (gRNA) targeting human GNB5 gene exon2 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods [ ] and confirmed via sequencing. .. Briefly, 10 μg of Px459 plasmid was digested with 5 μl (10 units) of BbsI restriction enzyme (NEB) for 5 h at 37 °C, run on 1% agarose gel and the digested product was then eluted with a gel extraction kit (Qiagen).

    Article Title: RGS11-CaMKII complex mediated redox control attenuates chemotherapy-induced cardiac fibrosis
    Article Snippet: Guide RNA (gRNA) targeting human RGS11 gene exon 15 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Article Title: A RGS7-CaMKII complex drives myocyte-intrinsic and myocyte-extrinsic mechanisms of chemotherapy-induced cardiotoxicity
    Article Snippet: Recipient cells were collected for further processing after 12-16 h. Generation of RGS7 KO AC-16 cells using CRISPR/Cas9 Guide RNA (gRNA) targeting human RGS7 gene exon17 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods as we previously described(2) and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Plasmid Preparation:

    Article Title: RGS11-CaMKII complex mediated redox control attenuates chemotherapy-induced cardiac fibrosis.
    Article Snippet: Guide RNA (gRNA) targeting human RGS11 gene exon 15 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Article Title: G protein β5-ATM complexes drive acetaminophen-induced hepatotoxicity
    Article Snippet: Guide RNA (gRNA) targeting human GNB5 gene exon2 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods [ ] and confirmed via sequencing. .. Briefly, 10 μg of Px459 plasmid was digested with 5 μl (10 units) of BbsI restriction enzyme (NEB) for 5 h at 37 °C, run on 1% agarose gel and the digested product was then eluted with a gel extraction kit (Qiagen).

    Article Title: RGS11-CaMKII complex mediated redox control attenuates chemotherapy-induced cardiac fibrosis
    Article Snippet: Guide RNA (gRNA) targeting human RGS11 gene exon 15 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).

    Article Title: A RGS7-CaMKII complex drives myocyte-intrinsic and myocyte-extrinsic mechanisms of chemotherapy-induced cardiotoxicity
    Article Snippet: Recipient cells were collected for further processing after 12-16 h. Generation of RGS7 KO AC-16 cells using CRISPR/Cas9 Guide RNA (gRNA) targeting human RGS7 gene exon17 were designed using tools available from Integrated DNA technologies (IDT, Newark, NJ, USA). .. High on target and low off target gRNAs were chosen without a PAM sequence, cloned into the PX459 CRISPR system plasmid (Addgene) using standard methods as we previously described(2) and confirmed via sequencing. .. The resulting construct was transfected into AC-16 cells using lipofectamine 3000 (Thermo Fisher).



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